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Overview

Microlyte® Matrix is a synthetic resorbable wound matrix that comprises a polyelectrolyte multilayer (PEM) nanofilm of cationic polyallylamine hydrochloride and anionic polyacrylic acid, which together act as a functional molecular template to facilitate the granulation in the wound bed. The nanofilm matrix​ is coated with a 20-micron layer of polyvinyl alcohol (PVA) to provide moisture management and product handleability. The nanofilm matrix contains a low level of ionic and metallic silver (<25µg/sqcm) to prevent microbial contamination and colonization of the matrix. This report summarizes the efficacy of silver-loaded PEMs on wound closure and provides a brief description of why Microlyte® Matrix works so well in assisting wound healing.​

The Polyelectrolyte Multilayer Matrix

Figure 1. The PEMs which comprise Microlyte® Matrix act as a scaffold to support mammalian cell growth. A) As expected, cells grow on a glass surface (not treated with silver-loaded PEMs). B) Interestingly, the same cells spread more easily on silver-loaded PEMS. This suggests that skin cells may grow more evenly on silver-loaded PEMS leading to re-epithelialization of a wound. ​

The PEMs Matrix and Wound Healing

Figure 2. Mouse skin cells grow on silver-treated PEMs leading to re-epithelialization of a full-thickness wound. (A-C) Mouse wounds treated with Microlyte® Matrix close faster and more completely compared to control PEMs without silver or untreated wounds. (D) Quantification of wound closure over time.

Why are silver loaded PEMs so effective?

Figure 3.

  • The PEM component of Microlyte® Matrix contains both anionic and cationic charged polymeric components which serve to shield approaching granulation cells from the generally disorganized surface chemistry common to wound beds.
  • This masking provides a functional molecular matrix or template to facilitate the granulation process in the healing wound.​
  • The structure and charge of the PEMs allows for a low, but precise level of silver to be incorporated into the multilayer to provide gentle, but effective antimicrobial activity in the wound.​
  • The hydrophilic and biocompatible polyvinyl alcohol (PVA) in Microlyte® Matrix maintains a physiologically moist microenvironment in the wound, which further facilitates wound healing.

Figure 4. Microlyte® Matrix absorbs into the wound, changing the terrain within the wound bed. Microlyte® Matrix (left) adheres to the wound bed, right where its matrix function is needed, while conventional matrices sit above the wound bed leaving space for microbes to hide and potentially flourish. Microlyte® Matrix creates a keratinocyte- and fibroblast-friendly environment that allows for faster and more complete wound closure.

Ref: Agarwal, Ankit, et al. Biomaterials 31.4 (2010): 680-690. Herron, Maggie, et al. Advanced healthcare materials 3.6 (2014): 916-928.

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